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Attogene – USA distributor for HighQu

HighQu has premium research tools that support life science worldwide with professionally simple, premium quality, outperforming products for end-point PCR, real-time PCR (qPCR), RT-PCR, cloning, electrophoresis, protein research, RNA analysis & other molecular biology applications. 

 

highQu supports research and development of SARS-CoV-2 detection tests.

1Step RT PCR Kit

$679.25

Applications

  • One step RT-PCR
  • RT-PCR of complex GC/TA rich templates
  • Fast RT-PCR

 

Benefits

  • Includes both RT Mix and RT-PCR Mastermix for RT and PCR in one tube
  • RT Mix contains RNase Inhibitor and thermostable RT (up to 55°C)
  • RT PCR Mastermix allows for low copy number targets detection

PCR Water

$38.35

Applications

  • PCR and qPCR
  • Other molecular biology applications

 

Benefits

  • Pure PCR-grade, nuclease-free water
  • Tested in most demanding PCR and qPCR applications

ORA™ qPCR Probe Mix, 2X

$152.75$688.35

Applications

  • qPCR assays based on specific probes:
  • TaqMan®, Molecular Beacons, Scorpions™ Probes
  • Gene expression analysis, quantification of:
  • gDNA, cDNA, viral DNA, low copy number genes

 

Benefits

  • Universal – both standard and fast cycling, all probe qPCR assays
  • qPCR of GC or AT rich templates, single-plex & multiplexing
  • Rapid extension, early Ct
  • Supplied with PCR Water
  • Bulk quantities & custom packaging available

ORA™ qPCR Probe ROX L Mix, 2X

$152.75$688.35

Applications

  • qPCR on instruments calibrated with low ROX concentration
  • qPCR assays based on specific probes:
  • TaqMan®, Molecular Beacons, Scorpions™ Probes
  • Gene expression analysis, quantification of:
  • gDNA, cDNA, viral DNA, low copy number genes

 

Benefits

  • Universal – both standard and fast cycling, all probe qPCR assays
  • qPCR of GC or AT rich templates, single-plex & multiplexing
  • Rapid extension, early Ct
  • Supplied with PCR Water
  • Bulk quantities & custom packaging available

ORA™ qPCR Probe ROX H Mix, 2X

$152.75$688.35

Applications

  • qPCR on instruments calibrated with low ROX concentration
  • qPCR assays based on specific probes:
  • TaqMan®, Molecular Beacons, Scorpions™ Probes
  • Gene expression analysis, quantification of:
  • gDNA, cDNA, viral DNA, low copy number genes

 

Benefits

  • Universal – both standard and fast cycling, all probe qPCR assays
  • qPCR of GC or AT rich templates, single-plex & multiplexing
  • Rapid extension, early Ct
  • Supplied with PCR Water
  • Bulk quantities & custom packaging available

ORA™ SEE qPCR Probe Mix, 2X

$152.75$688.35

Applications

  • qPCR assays based on specific probes:
  • TaqMan®, Molecular Beacons, Scorpions™ Probes
  • Gene expression analysis, quantification of:
  • gDNA, cDNA, viral DNA, low copy number genes

 

Benefits

  • Universal – both standard and fast cycling, all probe qPCR assays
  • qPCR of GC or AT rich templates, single-plex & multiplexing
  • Rapid extension, early Ct
  • Inert blue dye for a better sample visibility and tracking
  • Bulk quantities & custom packaging available

ORA™ SEE qPCR Probe ROX L Mix, 2X

$152.75$688.35

Applications

  • qPCR on instruments calibrated with low ROX concentration
  • qPCR assays based on specific probes:
  • TaqMan®, Molecular Beacons, Scorpions™ Probes
  • Gene expression analysis, quantification of:
  • gDNA, cDNA, viral DNA, low copy number genes

 

Benefits

  • Universal – both standard and fast cycling, all probe qPCR assays
  • qPCR of GC or AT rich templates, single-plex & multiplexing
  • Rapid extension, early Ct
  • Inert blue dye for a better sample visibility and tracking
  • Bulk quantities & custom packaging available

ORA™ SEE qPCR Probe ROX H Mix, 2X

$152.75$688.35

Applications

  • qPCR on instruments calibrated with high ROX concentration
  • qPCR assays based on specific probes:
  • TaqMan®, Molecular Beacons, Scorpions™ Probes
  • Gene expression analysis, quantification of:
  • gDNA, cDNA, viral DNA, low copy number genes

 

Benefits

  • Universal – both standard and fast cycling, all probe qPCR assays
  • qPCR of GC or AT rich templates, single-plex & multiplexing
  • Rapid extension, early Ct
  • Inert blue dye for a better sample visibility and tracking
  • Bulk quantities & custom packaging available

ORA™ qPCR Green ROX L Mix, 2X

$152.75$688.35

Applications

  • Dye based qPCR on instruments calibrated with low ROX concentration
  • qPCR from gDNA, cDNA, viral DNA, low copy number genes
  • Relative gene expression analysis, absolute quantification

 

Benefits

ORA™ qPCR Green ROX H Mix, 2X

$152.75$688.35

Applications

  • Dye based qPCR on instruments calibrated with high ROX concentration
  • qPCR from gDNA, cDNA, viral DNA, low copy number genes
  • Relative gene expression analysis, absolute quantification

 

Benefits

ORA™ qPCR HRM Mix, 2X

$152.74$688.35

Applications

  • High Resolution Melting analysis (HRM):
  • Detection of sequence variations
  • SNP genotyping
  • Methylation analysis
  • Mutation scanning

Benefits

  • Time and costs saving analysis of sequence variations
  • Universal – standard or fast cycling, GC or AT rich templates
  • Highest sensitivity, no optimization required
  • Supplied with PCR Water for maximum convenience
  • Bulk quantities & custom packaging available

ORA™ SEE qPCR Green ROX H Mix, 2X

$152.75$688.75

Applications

  • Dye based qPCR on instruments calibrated with high ROX concentration
  • qPCR from gDNA, cDNA, viral DNA, low copy number genes
  • Relative gene expression analysis, absolute quantification

Benefits

  • Universal – both standard and fast cycling
  • Excellent for GC or AT rich templates
  • Highest sensitivity, rapid extension, early Ct values
  • Inert blue dye for a better sample visibility and tracking
  • Bulk quantities & custom packaging available

ORA™ SEE qPCR Green ROX L Mix, 2X

$152.75$688.35

Applications

  • Dye based qPCR on instruments calibrated with low ROX concentration
  • qPCR from gDNA, cDNA, viral DNA, low copy number genes
  • Relative gene expression analysis, absolute quantification

Benefits

  • Universal – both standard and fast cycling
  • Excellent for GC or AT rich templates
  • Highest sensitivity, rapid extension, early Ct values
  • Inert blue dye for a better sample visibility and tracking
  • Bulk quantities & custom packaging available

1Step RT qPCR Probe Kit

$291.85$1,266.85

Applications

  • RT-qPCR assays based on specific probes:
  • TaqMan®, Molecular Beacons, Scorpions™ Probes
  • Gene expression analysis, quantification of:
  • gDNA, cDNA, viral DNA, low copy number genes

 

Benefits

  • Efficient Reverse transcription & sensitive qPCR in one tube
  • Both standard or fast cycling, GC rich templates
  • Rapid extension, early Ct
  • Supplied with PCR Water

1Step RT qPCR Probe ROX L Kit

$291.85$1,266.85

Applications

  • RT-qPCR on instruments requiring low ROX concentration
  • RT-qPCR assays based on specific probes:
  • TaqMan®, Molecular Beacons, Scorpions™ Probes
  • Gene expression analysis, quantification of:
  • gDNA, cDNA, viral DNA, low copy number genes

 

Benefits

  • Efficient Reverse transcription & sensitive qPCR in one tube
  • Both standard and fast cycling, GC rich templates
  • Rapid extension, early Ct
  • Supplied with PCR Water

1Step RT qPCR Probe ROX H Kit

$291.85$1,266.85

Applications

  • RT-qPCR on instruments requiring high ROX concentration
  • RT-qPCR assays based on specific probes:
  • TaqMan®, Molecular Beacons, Scorpions™ Probes
  • Gene expression analysis, quantification of:
  • gDNA, cDNA, viral DNA, low copy number genes

 

Benefits

  • Efficient Reverse transcription & sensitive qPCR in one tube
  • Both standard and fast cycling, GC rich templates
  • Rapid extension, early Ct
  • Supplied with PCR Water

1Step RT qPCR Green ROX L Kit

$291.85$1,266.85

Applications

  • Dye-based RT-qPCR on instruments requiring low ROX concentration
  • Relative gene expression analysis
  • Quantification of mRNA, total RNA, viral RNA, low copy number genes

 

Benefits

  • Efficient Reverse transcription & sensitive qPCR in one tube
  • Both standard and fast cycling, GC rich templates
  • Rapid extension, early Ct
  • Supplied with PCR Water

1Step RT qPCR Green ROX H Kit

$291.85$1,266.85

Applications

  • Dye-based RT-qPCR on instruments requiring high ROX concentration
  • Relative gene expression analysis
  • Quantification of mRNA, total RNA, viral RNA, low copy number genes

 

Benefits

  • Efficient Reverse transcription & sensitive qPCR in one tube
  • Both standard and fast cycling, GC rich templates
  • Rapid extension, early Ct
  • Supplied with PCR Water

ALLin™ Taq DNA Polymerase

$207.35$935.35

Applications

  • Routine PCR up to 6 kb
  • PCR of GC rich templates
  • Colony PCR, Fast PCR, TA cloning

 

Benefits

  • Higher yields under standard and fast cycling
  • Increased success in amplification of longer (6 kb) and GC rich templates
  • 5X ALLin™ PCR Buffer contains optimal Mg2+ and dNTP concentrations

ALLin™ Taq Mastermix, 2X

$129.35$584.35

Applications

  • Routine PCR up to 6 kb
  • PCR of GC rich templates
  • Colony PCR, Fast PCR, TA cloning

 

Benefits

ALLin™ Red Taq Mastermix, 2X

$129.35$584.35

Applications

  • Routine PCR up to 6 kb with a direct gel loading option
  • PCR of GC rich templates
  • Colony PCR, Fast PCR, TA cloning

 

Benefits

Taq DNA Polymerase

$324.35$584.35

Applications

  • Routine PCR up to 5 kb under common and fast conditions
  • RT-PCR, colony PCR,TA cloning
  • Library construction, genotyping, screening

 

Benefits

  • High yields in routine PCR and under fast cycling conditions
  • Robust performance under variety of conditions
  • Excellent performance on complex (GC rich) templates

ALLin™ Hot Start Taq Polymerase

$389.35$1,754.35

Applications

  • Hot-start PCR up to 6 kb, Low copy target detection
  • PCR of complex (GC rich) templates
  • Fast PCR, multiplexing, TA cloning

 

Benefits

  • Higher yields, no background in standard or fast PCR
  • Success on longer (6 kb) or GC rich templates, in crude sample PCR
  • 5X ALLin™ PCR Buffer contains optimal Mg2+ and dNTPs

ALLin™ Hot Start Taq Mastermix, 2X

$246.35$1,108.25

Applications

  • Hot-start PCR up to 6 kb, low copy target detection
  • PCR of complex (GC rich) templates
  • Fast PCR, multiplexing, TA cloning

 

Benefits

ALLin™ HS Red Taq Mastermix, 2X

$246.35$1,108.25

Applications

  • Hot-start PCR up to 6 kb with a direct gel loading option
  • Crude sample PCR
  • Low copy target detection
  • Amplification of complex (GC/AT rich) templates
  • Fast PCR
  • TA cloning
  • Multiplex hot-start PCR

 

Benefits

  • Outperforming sensitivity & specificity – low copy number target detection and no background
  • Higher yields under standard and fast cycling
  • Increased sensitivity and success in amplification of longer templates (6 kb), robust amplification of GC rich templates
  • Premixed with the red dye and density reagents for direct loading on the gels after the PCR
  • Bulk quantities & custom packaging available

ALLin™ RPH Polymerase

$259.35$1,169.35

Applications

  • Long PCR (up to 35 kb) with higher fidelity
  • Amplification of complex (GC rich) templates
  • Crude sample PCR, multiplex PCR, TA cloning

 

Benefits

  • Long (up to 35 kb) high-fidelity (5X higher than Taq) amplification
  • High yields in standard and fast PCR, on GC rich DNA and crude samples
  • 5X ALLin™ PCR Buffer contains optimal Mg2+ and dNTPs

ALLin™ HiFi DNA Polymerase

$194.35$874.25

Applications

  • High-fidelity PCR up to 10 kb
  • Complex (GC rich) template or crude sample PCR
  • Fast PCR
  • Blunt cloning

 

Benefits

  • 50 x higher fidelity than classic Taq
  • High sensitivity and yields under standard and fast cycling
  • Increased success in long (10 kb) or GC rich PCR
  • 5X ALLin™ Buffer contains optimal Mg2+ and dNTPs

ALLin™ RPH Mastermix, 2X

$328.25$1,481.25

Applications

  • Long PCR (up to 35 kb) with higher fidelity
  • Amplification of complex (GC rich) templates
  • Crude sample PCR, multiplex PCR, TA cloning

 

Benefits

  • Long (up to 35 kb) high-fidelity (5X higher than Taq) amplification
  • High yields in standard and fast PCR, on GC rich DNA and crude samples
  • ALLin™ mastermix supplied with PCR Water
  • Bulk quantities & custom packaging available

SampleIN™ Direct PCR Kit

$127.40$518.70

Applications

  • Fast direct PCR without template purification
  • Mouse genotyping and knockout analysis
  • Direct PCR from mouse tail or ear, mammalian tissues (including FFPE)
  • Direct PCR from hair follicle, buccal swabs, plant samples
  • Direct Blood PCR (including EDTA or FTA samples)

 

Benefits

  • Ready-to load PCR in 50 minutes without template purification
  • Single-tube 15 min DNA extraction combined with fast hot-start PCR
  • Red dye in the PCR master mix for direct gel loading
  • High yields under standard or fast cycling conditions
  • Success with GC/AT rich templates

qScriber™ cDNA Synthesis Kit

$155.35$506.35

Applications

  • cDNA template generation for qPCR or PCR
  • Unbiased, efficient cDNA synthesis
  • Detection of low target amounts
  • cDNA synthesis from complex templates

 

Benefits

  • Thermostable HighScriber™Reverse Transcriptase blended with Ribonuclease Inhibitor for efficient cDNA synthesis
  • Optimized reaction mix with oligo (dT) and random primers for unbiased representation of mRNA ends
  • cDNA synthesis from complex templates at up to 55°C
  • High sensitivity detection from 1 pg of total RNA template

UDGin™ PCR Cleaner Mix, 20X

$63.70

Applications

  • Efficient prevention of carry-over contamination in qPCR
  • Efficient prevention of carry-over contamination in PCR

 

Benefits

  • Time saving ready-to-use mix with UDG and dUTP
  • Compatible with all highQu qPCR and PCR master mixes

PCRbeam™ Fast PCR Detection Kit

$155.35

Applications

  • Low throughput PCR, LAMP, RPA based tests
  • Sensitive detection of specific amplification products
  • Fast and 20x more sensitive alternative to EtBr stained gels
  • Economical alternative to qPCR-based detection

 

Benefits

  • Sensitive detection of PCR, LAMP, RPA gene-specific products
  • No gel loading after PCR, no ethidium bromide handling
  • Saved costs compared to qPCR-based detection methods
  • Fast and easy procedure with little hands on time

25 mM dNTP Mix

$259.35

Applications

  • Standard PCR, long and high-fidelity PCR
  • cDNA synthesis, RT-PCR, qPCR
  • Sequencing

 

Benefits

  • Highest quality, >99% HPLC pure dNTPs
  • Free of PCR inhibitors
  • Highly stable

10 mM dNTP Mix

$120.25

Applications

  • Standard PCR, long and high-fidelity PCR
  • cDNA synthesis, RT-PCR, qPCR
  • Sequencing

 

Benefits

  • Highest quality, >99% HPLC pure dNTPs
  • Free of PCR inhibitors
  • Highly stable

100 mM dNTP Set

$168.35

Applications

  • Standard PCR, long and high-fidelity PCR
  • cDNA synthesis, RT-PCR, qPCR
  • Sequencing

 

Benefits

  • Highest quality, >99% HPLC pure dNTPs
  • Free of PCR inhibitors
  • Highly stable

Rally™ Rapid Ligation Kit

$155.35$623.35

Applications

  • Cloning, vector-insert ligation
  • Adaptor or linker ligation
  • Linear DNA self-circularization

 

Benefits

  • 5 minutes fast high efficiency ligation
  • Universal for both blunt or cohesive-end ligations
  • Premium reagents – reproducible results

Take5™ 1kb DNA Ladder

$126.75

Applications

  • DNA sizing on agarose gels
  • Approximate DNA quantification on agarose gels

 

Benefits

  • Room-temperature-stable ladders – always ready to use
  • Sharp bands, bright reference bands, indicated DNA mass
  • Supplied with loading dye for DNA samples

HighEnd™ Repair Kit

$168.35$701.35

Applications

  • Preparation of PCR products, sheared or nebulized DNA, restriction-digested DNA, cDNA for blunt-end ligation
  • Conversion of 5′- and/or 3′-protruding ends to 5′-phosphorylated blunt-ended ones

 

Benefits

  • Fast and simple blunting and phosphorylation of DNA at once
  • Universal – preparing any kind of DNA for blunt-end ligation
  • Premium reagents – reproducible results

Take5™ 100 bp DNA Ladder

$126.75

Applications

  • DNA sizing on agarose gels
  • Approximate DNA quantification on agarose gels

 

Benefits

  • Room-temperature-stable ladders – always ready to use
  • Sharp bands, bright reference bands, indicated DNA mass
  • Supplied with loading dye for DNA samples

Take5™ 50 bp DNA Ladder

$142.35

Applications

  • DNA sizing on agarose gels
  • Approximate DNA quantification on agarose gels

 

Benefits

  • Room-temperature-stable ladders – always ready to use
  • Sharp bands, bright reference bands, indicated DNA mass
  • Supplied with loading dye for DNA samples

Take5™ HR DNA Ladder

$142.35

Applications

  • DNA sizing on agarose gels
  • Approximate DNA quantification on agarose gels

 

Benefits

  • Room-temperature-stable ladders – always ready to use
  • Sharp bands, bright reference bands, indicated DNA mass
  • Supplied with loading dye for DNA samples

Cozy™ Prestained Protein Ladder

$259.35

Applications

  • Approximate protein molecular weight determination on denaturing gels
  • Monitoring of electrophoresis process and Western transfer efficiency

 

Benefits

  • Room-temperature-stable ladders, always ready to use
  • Sharp bands, bright colors

CozyHi™ Prestained Protein Ladder

$272.35

Applications

  • Approximate protein molecular weight determination on denaturing gels
  • Monitoring of electrophoresis process and Western transfer efficiency

 

Benefits

  • Room-temperature-stable ladders, always ready to use
  • Sharp bands, bright colors

CozyXL™ Prestained Protein Ladder

$324.35

Applications

  • Approximate protein MW determination on denaturing gels
  • Monitoring of electrophoresis process and Western transfer efficiency

 

Benefits

  • Room-temperature-stable ladders, always ready to use
  • Sharp bands, bright colors

StainIN™ RED Nucleic Acid Stain

$127.40

Applications

  • Staining of NA in agarose and polyacrylamide gels during electrophoresis for ssDNA, dsDNA and RNA visualization and gel documentation under the UV light

 

Benefits

  • Much more safe alternative to ethidium bromide, more economical compared to other dyes
  • Highly sensitive NA detection – up to 2x more sensitive than EtBr
  • Time saving – in gel stain, no post- run staining, no destaining

StainIN™ GREEN Nucleic Acid Stain

$127.40

Applications

  • Staining of NA in agarose and polyacrylamide gels during electrophoresis for ssDNA, dsDNA and RNA visualization and gel documentation
  • Blue LED detection, excellent for cloning applications
  • UV detection

 

Benefits

  • Much more safe alternative to ethidium bromide, more economical alternative to competing green dyes
  • Unique – has two emission peaks, colors DNA in green, RNA in red
  • Highly sensitive NA detection – up to 4x more sensitive than EtBr
  • Time saving – in gel stain, no post- run staining, no destaining

HighScriber™ Reverse Transcriptase Mix, 20X

$246.35$857.35

Applications

  • cDNA synthesis of up to 15 kb long transcripts
  • Template generation for RT-PCR & RT-qPCR
  • cDNA synthesis from complex templates

 

Benefits

  • Thermostable Reverse Transcriptase blended with Ribonuclease Inhibitor for efficient cDNA synthesis
  • High yields of full lengths transcripts up to 12-15 kb
  • cDNA synthesis from complex templates at up to 55°C
  • High sensitivity detection from 1 pg of total RNA template

SecurRIN™ Advanced RNase Inhibitor

$96.85$389.35

Applications

  • Prevention of RNA degradation during:
  • cDNA synthesis, RNA extraction & storage
  • in vitro transcription and translation
  • Monoclonal antibody preparation procedures

Benefits

  • Efficient RNAse inhibition even at higher temperatures up to 60°C
  • RNA protection from hydrolysis by RNases A, B and C
  • Economical & stable for weeks at ambient temperature
  • Robust, works well at 5.5 to 9.0 pH, with 0.5 – 1 mM DTT
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