1Step RT qPCR Probe ROX H Kit


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  • RT-qPCR on instruments requiring high ROX concentration
  • RT-qPCR assays based on specific probes:
  • TaqMan®, Molecular Beacons, Scorpions™ Probes
  • Gene expression analysis, quantification of:
  • gDNA, cDNA, viral DNA, low copy number genes



  • Efficient Reverse transcription & sensitive qPCR in one tube
  • Both standard and fast cycling, GC rich templates
  • Rapid extension, early Ct
  • Supplied with PCR Water
SKU: QOP030 Category: Tag:

highQu 1Step RT qPCR mastermixes in combination with a blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT Mix) allow for a single step one tube RT qPCR.

qPCR mastermixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT/GC rich templates and guaranty rapid extension with early Ct values with minimum or no optimization.

Our mastermixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the 1Step RT qPCR Probe Mixes are available in three versions – without ROX, with low or high ROX concentration.

For optional use, the ROX passive reference dye is premixed within the ROX L and ROX H qPCR Mixes. If the purchaser has an instrument capable of optional ROX detection and wishes to perform the optional normalization of the signal, then the user must select the option in the software.
Notice to Purchaser: With purchasing of this product, no rights are conveyed with respect to U.S. Patent: 5,928,907 and corresponding patents outside the US.

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