Alkaline Phosphatase Assay Kit

$295.00

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Highly Sensitive, rapid, robust screening kit for sample phosphatase activity.

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Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.

Features and Benefits

  • High sensitivity and wide linear range. The detection limit is 2 U/L, linear up to 800 U/L.
  • Simple “mix-and-measure” procedure allows reliable quantitation of ALP activity within 5 minutes.
  • Compatible with high-throughput handling systems.

Suitability

The kit is suitable for the quantitative determination of alkaline phosphatase (ALP) activity in serum, plasma etc.

Principle

The Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at A405 nm). The rate of the reaction isdirectly proportional to the enzyme activity.

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