Piscine reovirus (PRV)


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Product features

  • • Exceptional value for money
  • • Rapid detection of all clinically relevant subtypes
  • • Positive copy number standard curve for quantification
  • • Highly specific detection profile
  • • High priming efficiency
  • • Broad dynamic detection range (>6 logs)
  • • Sensitive to < 100 copies of target
  • • Accurate controls to confirm findings
SKU: Path-PRV-standard Category: Tag:

Piscine orthoreovirus (PRV) belongs to the Reoviridae family and is the only known fish virus related to the Orthoreovirus genus. The virus is the causative agent of heart and skeletal muscle inflammation (HSMI), an emerging disease in farmed Atlantic salmon. PRV is ubiquitous in farmed Atlantic salmon and high loads of PRV in the heart are consistent findings in HSMI.
PRV has a segmented, double-stranded RNA (dsRNA) genome and belongs to the Reoviridae family. PRV has 10 genomic segments, as do the orthoreoviruses, but the overall amino acid identity between the homologous proteins is very low, particularly for the surface-exposed and non-structural proteins. However, several amino acid motifs central to protein function are conserved for orthoreoviruses and PRV.
Although high loads of PRV in the heart are a consistent finding in HSMI outbreaks, the virus can be detected at low levels in fish throughout the production cycle. PRV has also been detected in farmed Atlantic salmon in Canada and Chile, farmed steelhead trout (Oncorhynchus mykiss), wild chum salmon (O. keta) in Canada and in wild Atlantic salmon and brown trout (Salmo trutta L.) in Norway.
In aquaculture farming, large numbers of animals are kept confined at high densities allowing for the rapid proliferation of the infectious agents.
HSMI was first described in Norway in 1999 . HSMI is mainly observed during the seawater grow-out phase of the fish, with morbidity close to 100% in affected cages, while cumulative mortality varies from negligible to 20%.
Typical gross pathologic changes in affected fish include signs of circulatory disturbance; pale heart, yellow liver, swollen spleen and petechiae in perivascular fat. (blood spots in the fat around the heart)
Diagnosis of HSMI is currently based on typical histopathological findings in heart and red skeletal muscle. Real-Time PCR enables the non-veterinary pathologist to detect the virus and offer a reliable diagnosis without need for extensive post-mortem examinations. By explicitly targeting a conserved Non-structural RNA Protein gene, this genesig® ensures high discrimination towards PRV and other, genetically similar samples.
Real-Time PCR remains the most rapid, economic and reliable way to detect PRV in samples.

Piscine reovirus Manual