High Resolution Melting analysis (HRM) is a fast and simple technique for identification of DNA sequence variations. It allows identifying single nucleotide differences by detecting minor changes in qPCR melting curves.
highQu ORA™ HRM qPCR Mix includes a proprietary intercalating saturating dye showing no inhibition for PCR. The dye has the same affinity for both AT or GC rich sequences what leads to highest accuracy in genotyping.
The hot-start function in the mix is based on the small molecular inhibitor technology and allows achieving highest sensitivity and specificity under both standard and fast qPCR cycling conditions. The mix provides excellent performance on both AT and GC rich templates and reliable results with minimum or no optimization.
Our mastermixes are supplied with PCR Water to guaranty the best performance.