Recombinant SARS CoV-2 nucleocapsid protein (antigen) is coated onto 96-well plates. The controls, test samples and Biotin-labeled antigen were added to the wells and washed. HRP-Streptavidin Conjugate is next added and unbound conjugates are washed. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding the stop solution. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650 nm.
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