This kit is a sandwich enzyme-linked immunesorbent assay that detects the SARS CoV-2 nucleoprotein. The capture antibody (monoclonal) is coated onto 96-well plates. A biotin conjugated antibody is used as a detection antibody. The standards, test samples and biotin conjugated detection antibody are added to the wells and washed. Next, HRP-Streptavidin is added and unbound conjugates are washed. The TMB substrate is catalyzed by HRP to produce a blue color product that changes to yellow after adding the stop solution. The intensity of color change is proportional to the target amount in the sample. The absorbance at 450nm can be used to calculate the concentration of target.
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