highQu Taq DNA Polymerase is the classical enzyme for routine PCR applications providing high amplification yields of 3-5 kb targets under various conditions. Taq DNA Polymerase is purified from a recombinant E. coli strain carrying the Taq DNA polymerase gene.
Taq DNA polymerase is thermostable 5´ → 3´ DNA polymerase. It lacks 3´ → 5´ exonuclease (proofreading) activity and has low 5´ → 3´ exonuclease activity. Polymerase exhibits deoxynucleotidyl transferase activity resulting in A-overhang at the 3′-ends of PCR products and allowing for TA cloning. The PCR accuracy of Taq DNA Polymerase is 4.5 x 104 (a number of incorporated nucleotides before the first error occurs).
The supplied reaction buffer for most flexibility in optimization, contains no dNTPs and no magnesium. The 50 mM MgCl2 solution is provided separately what allows for magnesium optimization starting from 1 mM up to 4 mM final concentration upon the need. The dNTPs in sets or mixes can be purchased from highQu separately.