FAM and Biotin End Labeled 23S 874bp Amplicon fragment NALFIA control DNA

FAM and Biotin End-labeled 23S Amplicon fragment of 874 base pairs – Derived from a strain of Microcystis aeruginosa.  Amplification was performed using a set of specific primers:

Forward primer: 5’- /5Biosg/AGGGGTAAAGCACTGTTTCG -3′ 20ul of a 10uM solution

Reverse primer: 5’- /56-FAM/CCTTCTCCCGAAGTTACG -3′ 20ul of a 10uM solution

This PCR Amplicon is functionally tested to work with the Universal Nucleic Acid Lateral Flow ImmunoAssay (NALFIA) kit catalog number: AU2034 -01 and a perfect control to be used for a larger nucleic acid tagged amplified product with the Universal Lateral Flow systems.

Sequenced using mini ion with Microcystis aeruginosa UTEX #2388:

Results:

99.76% match: Microcystis aeruginosa NIES-933 gene for 23S rRNA, partial sequence

99.51% match: Microcystis aeruginosa UTEX LB 2388 23S ribosomal RNA gene, partial sequence

Sequence identified using Nanopore from gel purified PCR product produced using primer set used in attogene kit:

ATTTTTTTGTCCTCTACTGGTTCGGTGCTATGTAAAACGGGTGTTTAACCGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGT

GCGCCGCTTCAAGCACTGTTTCGTAACTTCGGGAGAAGGAGTAGGGGTAAAGCACTGTTTCGCCGCGGGCTGGGAGACCGGTACCAA

GGCGAGGCAAACTCTGAATACGGCTAAAAAAACTACCAGTGAGACGGTGGGGGATAAGCTTCATCGTCAAGAGGGAAACAGCCCAGA

CCACCAGCTAAGGTCCCCAAATGATAACTAAGTGAGAAAGGAGGTGGGAGTGCATTGACAACCAGGAGGTTTGCCTAGAAGCAGCAA

CCCTTAAAAGAGTGCGTAATAGCTCACTGGTCAAGCGCTCCTGCGCCGAAAATGAACGGGGCTAAGTTATCTACCGAAGCTGTGGACT

ACATTGTAGTGGTAGGGGAGCGTTCTATAGGGGGTGAAGCAGTAGCGGCAAGCAGCTGTGGACTCTATAGAAGTGAGAATGTCGGCT

TAAGTAGCGAAAATGTGGGTGAGAATCCCACACCCCGAAACCCCAAGGTTTCCTCCCGCAAGGCTCGTCCGCGGAGGGTCAGTCAGGA

CCTAAGGCGAGGCTGAAAAGCGTAGTCGATGGACAACGGGTTAACATTCCCGTACCGATTAATGATTGTGCCGGAGAACGGAGAAGGT

CAACGTCAGCTGGATGTTGGTTACCAGTGCAAGCAGTCGAGGCGATGAGAGGTGGCGAAAACACCAGGAGCTAAGACGCGAGTCCCAC

CTCCCACGGGAGGGAAGTGGCGTTGATCAAGCTTCCTAGAAAAGCCCGAACCACGTTAATCATTAATTGCCTGTACCCGAAACCGACAC

AGGTGGGGAAGTAGAGAATACTAAGGGGCGCGAGATAACTCTCTCTAAGGAACTCGGCAAAATGACCCCGTAACTG

Perfect as a control for Attogene NALFA