The Okadaic plate kit is a competitive enzyme-labeled immunoassay. The Okadaic Acid sample extract and calibrators are pipetted into the test wells followed by the Okadaic Acid antibody into the test wells to initiate the reaction. During the 30 minutes incubation period, Okadaic Acid from the sample and Okadaic Acid antigen compete for binding to the Okadaic Acid antibody. The Okadaic Acid antibody is captured on the walls of the test well. Following this 30 minutes incubation, the contents of the wells are removed and the wells are washed to remove any unbound Okadaic Acid and free Okadaic Acid antibody. After wash, 1X HRP-conjugated Antibody#2 is added for 30 minutes incubation. The wells are washed afterwards, and a clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 15 minutes incubation, the reaction is stopped and the amount of color in each well is read. The color of the unknown samples is compared to the color of the calibrators and the Okadaic Acid concentration of the samples is derived.
Okadaic acid is the causative agent of Diarrhetic Shellfish Poisoning (DSP).
FDA and EPA Safety Levels in Regulations and Guidance – 0.16 mg/kg for Clams, mussels, oysters, and whole and roe-on scallops, fresh, frozen, or canned. – National Shellfish Sanitation Program Guide for the Control of Molluscan Shellfish.
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